Botany lab 13 Chromatography failure
The Chromatography laboratory failed spectacularly. Every single plate, with three different plate types, looked like this. The solvent above was 70% ethyl alcohol. The pasty white appearance is likely a result of too much calcium carbonate. Most of the class used 91% isopropyl alcohol with a result seen as above. Ethyl alcohol was used successful last year. 70% isopropyl alcohol was also used successfully last year. Perhaps the plant leave mix was problematic. Alternanthera sessifolia was the dominant plant in the mix. Some Codiaeum variegatum and Cordyline fruticosa leaves were added in. Last year only Alternanthera sessifolia was used. Was that contributory to the issues? The calcium carbonate was locally sourced. Was too much added? Not enough? About a half to one teaspoon was used. Perhaps there were impurities in the calcium carbonate. The use of an eye dropper tended to create larger blobs, but n...